Core
Areas
Biomechanics
Orthopaedic biomechanics and biomaterials testing using the MTS Bionix system The Bionix system is rated for 25 kN axial and 20 Nm torsional testing Three-point-loading Compressive and Tensile testing Load to failure Fatigue testing Photo of biomechanics lab
Decalcified and soft tissue paraffin slide preparation for samples to 80 x 60mm -
Undecalcified hard tissue slide preparations using JB4 and Exakt systems -
Static and dynamic histomorphometry -
Methyl methacrylate embedded sections -
Routine and special stains on above generated slides -
Closed system specimen radiography -
Image gallery
Bone Mineral Densitometry
Summary of Bone Mineral Densitometry Micro computed tomography (micro CT) using Scanco micro CT 40. Peripheral Quantitative Computed Tomography (pQCT) using a Norland Research SA+ Dual-Energy X-Ray Absorptiometry (DEXA) using the Lunar PIXImus Densitometer
Skeletal Phenotyping
The Center for Orthopaedic Research provides platform skeletal phenotyping for any transgenic and mutant mice generated or used at UAMS. The skeletal phenotype provides meaning to the genotype. Please contact us if you require any help with the analysis of the skeleton. (see recent example)
- Multi-level analysis of the skeleton
- Fetal skeleton staining
- X-ray; DEXA; pQCT; microCT
- Detailed histological and histomorphometric evaluation
This effort helps to ensure that:
1. No skeletal phenotypes,
independent of the manipulated gene of interest or procedure, are missed
2.
3. The value of the mice is returned to the PI and the University
Bioluminescent and Fluorescent Imaging
The Xenogen IVIS Imaging System is a highly sensitive CCD camera optimized for biophotonic imaging. UAMS researchers can use this single system to perform quantitative in vitro and in vivo assays using reporter cells tagged with both bioluminescence and/or fluorescence.
Light emitting probes, such as bioluminescent luciferases or fluorescent proteins, are commonly used as reporters of gene expression in living cells for the study of biological activity and the development of therapeutic agents. Both bioluminescent and fluorescent reporters can be detected and monitored in vivo.
The IVIS Imaging System acquires a photographic image of the mice overlayed with a bioluminescent or fluorescent image. The intensity of the bioluminescent or fluorescent image is displayed in a pseudocolor scale in which blue regions represent lower photon emission and red regions represent greatest photon emission.
For more information or to schedule time in the IVIS Lab, please email bhogue@uams.edu
Protein Biomarker Identification
The Center for Orthopaedic Research in collaboration with the Arkansas Breast Cancer Research Program has developed a core facility for protein biomarker identification using SELDI (surface-enhanced laser disorption/ionization patented and manufactured by Ciphergen). The focus of the UAMS SELDI facility is biomarker identification in cancer.
The SELDI resource is capable of:
- Clinical and Research Proteomics
- Biomarker identification and validation
- Protein discovery
- Protein characterization
